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KMID : 0881720160310030222
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Journal of Food Hygiene and Safety
2016 Volume.31 No. 3 p.222 ~ p.225
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Comparative Evaluation of Selective Chromogenic Media for Coliforms Bacteria Isolated from Food
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Lee Da-Yeon
Kim Hee-eon
Cho Yong-Sun
Lee Jin-Sung
Kim Keun-Sung
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Abstract
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In this study, the performance of five selective media for coliform bacteria was evaluated. In total, 83 coliform isolates from ready-to-eat food and 21 reference strains were inoculated in five agar media : Chromocult coliform agar (Merck Millipore), HiCrome coliform agar (Sigma), CHROMagar ECC chromogenic media, Brilliance E. coli/coliform selective agar (OXOID), and endo agar (Merck Millipore). Coliform isolates and reference strains were inoculated on the selective media to test media sensitivity and specificity. The tested media showed the following sensitivities for the isolated strains: Chromocult coliforms agar and HiCrome coliform agar, 94%; Brilliance E. coli/coliform selective agar, 93%; CHROMagar ECC chromogenic media, 92%; and endo agar, 74%. In addition, all media showed 100% specificity, except for endo agar (71%). Moreover Chromocult coliform agar and HICrome coliform agar showed high levels recovery. Taken together, these results identified Chromocult coliform agar and HICrome coliform agar as an effective selective medium for coliforms with higher sensitivity and specificity compared to other media tested in this study.
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KEYWORD
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Selective media, Coliforms, Chromogenic media, Evaluation
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